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primescripttm reverse transcriptase reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm reverse transcriptase reagent kit
    Primescripttm Reverse Transcriptase Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+reverse+transcriptase+reagent+kit/PrimeScript+Reverse+Transcriptase/pm42133204-232-8-13
    Average 97 stars, based on 4907 article reviews
    primescripttm reverse transcriptase reagent kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction
    Article Snippet: Total RNAs were extracted from the cells using Trizol Reagent (Sigma-Aldrich, USA). .. A total of 500 ng RNAs were subjected to reverse transcription using a PrimeScript reverse transcriptase reagent kit (TaKaRa, Japan), followed by regular PCR (RT-PCR) detection with the PCR Amplification Kit (TaKaRa, Japan) or quantitative real-time PCR (RT-qPCR) detection with the SYBR Premix Ex Taq kit (TaKaRa, Japan). ..

    Article Title: circ-UBR5 in hypoxia-induced exosomes may mediate lung adenocarcinoma metastasis via the targeting of HNRNPR
    Article Snippet: Total RNA was extracted from cultured cells and tissues with TRIzol reagent (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s protocols. .. Complementary DNA (cDNA) was reverse-transcribed from 500 ng of total RNA with the PrimeScript Reverse Transcriptase Reagent Kit (Takara Bio, Kusatsu, Japan). .. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed with SYBR Premix Ex TaqII (Takara Bio) on a Light Cycler 480 II real-time PCR system (Roche, Basel, Switzerland).

    Article Title: Programmed downregulation of METTL3 is essential for decidualization in both humans and mice
    Article Snippet: Genomic DNA was eliminated through treatment with DNase I (Invitrogen). .. The cDNA synthesis was achieved using the PrimeScript reverse transcriptase reagent kit (TaKaRa). .. Quantitative PCR reactions were performed on the Applied Biosystems 7500 platform (Life Technologies) with the THUNDERBIRD SYBR qPCR Mix (Toyobo).

    Article Title: Y10 phosphorylation of LDHA promotes the release of extracellular vesicle-derived circSEPT9 to enhance the chemoresistance of triple negative breast cancer cells via modulation of miR-515-5p/KIAA1429 axis.
    Article Snippet: Objective: Extracellular vesicle (EV)-derived RNAs play crucial roles in cancer biology and therapeutic resistance.. This study investigated how EV-derived circSEPT9 mediates doxorubicin (DOX) resistance in triple negative breast cancer (TNBC).. Methods: TNBC tissues and samples were analyzed alongside MDA-MB-231 and MDA-MB-231/ADR cells.

    Article Title: Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction.
    Article Snippet: Total RNAs were extracted from the cells using Trizol Reagent (Sigma-Aldrich, USA). .. A total of 500 ng RNAs were subjected to reverse transcription using a PrimeScript reverse transcriptase reagent kit (TaKaRa, Japan), followed by regular PCR (RT-PCR) detection with the PCR Amplification Kit (TaKaRa, Japan) or quantitative real-time PCR (RT-qPCR) detection with the SYBR Premix Ex Taq kit (TaKaRa, Japan). ..

    Article Title: TNFRSF12A expression in stomach adenocarcinoma and its preliminary role in predicting immunotherapy response
    Article Snippet: Total RNA of GES-1 and AGS cells was collected utilizing the TRIzol reagent (15596026, Thermo Fisher). .. Then, the PrimeScript reverse transcriptase reagent Kit (RR037Q, Takara, Japan) was used to synthesize cDNA. .. RT−qPCR was carried out using the SYBR Green Universal Master Mix (4364344, Thermo Fisher) in a QuantStudio 3 Real-Time PCR System (Thermo Fisher).

    Article Title: Participation of COX2/mPGES1/PGE2 in mouse and human endometrial stromal decidualization.
    Article Snippet: .. Then, the RNAs (500 ng) were reverse transcribed into cDNAs using the PrimeScript reverse transcriptase reagent kit (TaKaRa, Tokyo, Japan). .. The cDNAs were amplified using the SYBR Premix Ex Taq kit (TaKaRa; DRR041S) on the CFX96 Touch RealTime PCR Detection System (Bio-Rad, Hercules, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction
    Article Snippet: Total RNAs were extracted from the cells using Trizol Reagent (Sigma-Aldrich, USA). .. A total of 500 ng RNAs were subjected to reverse transcription using a PrimeScript reverse transcriptase reagent kit (TaKaRa, Japan), followed by regular PCR (RT-PCR) detection with the PCR Amplification Kit (TaKaRa, Japan) or quantitative real-time PCR (RT-qPCR) detection with the SYBR Premix Ex Taq kit (TaKaRa, Japan). ..

    Article Title: Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction.
    Article Snippet: Total RNAs were extracted from the cells using Trizol Reagent (Sigma-Aldrich, USA). .. A total of 500 ng RNAs were subjected to reverse transcription using a PrimeScript reverse transcriptase reagent kit (TaKaRa, Japan), followed by regular PCR (RT-PCR) detection with the PCR Amplification Kit (TaKaRa, Japan) or quantitative real-time PCR (RT-qPCR) detection with the SYBR Premix Ex Taq kit (TaKaRa, Japan). ..

    Amplification:

    Article Title: Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction
    Article Snippet: Total RNAs were extracted from the cells using Trizol Reagent (Sigma-Aldrich, USA). .. A total of 500 ng RNAs were subjected to reverse transcription using a PrimeScript reverse transcriptase reagent kit (TaKaRa, Japan), followed by regular PCR (RT-PCR) detection with the PCR Amplification Kit (TaKaRa, Japan) or quantitative real-time PCR (RT-qPCR) detection with the SYBR Premix Ex Taq kit (TaKaRa, Japan). ..

    Article Title: Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction.
    Article Snippet: Total RNAs were extracted from the cells using Trizol Reagent (Sigma-Aldrich, USA). .. A total of 500 ng RNAs were subjected to reverse transcription using a PrimeScript reverse transcriptase reagent kit (TaKaRa, Japan), followed by regular PCR (RT-PCR) detection with the PCR Amplification Kit (TaKaRa, Japan) or quantitative real-time PCR (RT-qPCR) detection with the SYBR Premix Ex Taq kit (TaKaRa, Japan). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction
    Article Snippet: Total RNAs were extracted from the cells using Trizol Reagent (Sigma-Aldrich, USA). .. A total of 500 ng RNAs were subjected to reverse transcription using a PrimeScript reverse transcriptase reagent kit (TaKaRa, Japan), followed by regular PCR (RT-PCR) detection with the PCR Amplification Kit (TaKaRa, Japan) or quantitative real-time PCR (RT-qPCR) detection with the SYBR Premix Ex Taq kit (TaKaRa, Japan). ..

    Article Title: Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction.
    Article Snippet: Total RNAs were extracted from the cells using Trizol Reagent (Sigma-Aldrich, USA). .. A total of 500 ng RNAs were subjected to reverse transcription using a PrimeScript reverse transcriptase reagent kit (TaKaRa, Japan), followed by regular PCR (RT-PCR) detection with the PCR Amplification Kit (TaKaRa, Japan) or quantitative real-time PCR (RT-qPCR) detection with the SYBR Premix Ex Taq kit (TaKaRa, Japan). ..

    cDNA Synthesis:

    Article Title: Programmed downregulation of METTL3 is essential for decidualization in both humans and mice
    Article Snippet: Genomic DNA was eliminated through treatment with DNase I (Invitrogen). .. The cDNA synthesis was achieved using the PrimeScript reverse transcriptase reagent kit (TaKaRa). .. Quantitative PCR reactions were performed on the Applied Biosystems 7500 platform (Life Technologies) with the THUNDERBIRD SYBR qPCR Mix (Toyobo).

    other:

    Article Title: Deciphering the genetic basis of sex differentiation in silver-lipped pearl oyster ( Pinctada maxima ) based on integrative transcriptomic analysis
    Article Snippet: The purified RNA subsequently served as a template for reverse transcription using a PrimeScriptTM Reverse Transcriptase Reagent Kit (Takara, China), following the manufacturer’s guidelines.



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